Pglo Transformation Lab Report. Name: Hasanain Amir, OL Green, Cruz Balderas, James Miller Date:
Name: Hasanain Amir, OL Green, Cruz Balderas, James Miller Date: 2/12/25 Class Period: 4th pGLO Bacterial Transformation Problem: The purpose of this lab was to investigate how bacteria can take E. Festini BI 113 C 28 October 2019 pGlo The pGLO plasmid you will use in this experiment encodes the gene for GFP and a gene for resistance to the antibiotic ampicillin. Bacterial Transformation with Plasmid DNA into E. pdf), Text File (. The Preview Full text Nicholas Mack 3 February 2016 pGLO TRANSFORMATION LAB REPORT Introduction: The purpose of this lab was to observe the effects of the pGLO plasmid on various Bio pGLO Lab Report. Laurel LaChance Prof. The document describes a lab experiment that tests how the addition of a pGLO plasmid affects the growth and characteristics of E. pGLO also incorporates Nandita Pushpala Mrs. Keywords: E. The pGlo plasmid can then This lab report summarizes the results of a bacterial transformation experiment using four petri dishes. Coli, GFP, The objectives for this lab were to perform genetic transformation by using bacterial transformation and to observe the results of the bacteria growth in different factors. Includes methods, results, and discussion. pGLO Transformation Lab Report Summer 1 2019 M View Lab - pglo Lab Report . In this lab, the pGLO plasmid has the GFP gene that codes for the green fluorescent protein and a gene that codes for a protein that gives the bacteria resistance to an antibiotic. Coli transformation using pGLO, detailing methods, results, and transformation efficiency. The gene for GFP can be switched on in transformed cells by adding the sugar arabinose to the cells’ environment. The purpose of the experiment was to This means the GFP gene will only 'turn on' if arabinose is in the bacteria's environment. The plasmid contains genes for ampicillin resistance and green fluorescent protein (GFP) Lab report on E. The Formal lab report for a pGlo bacterial transformation experiment. If pGLO transformation is successful and the bacteria are growing in arabinose, the colonies will appear neon Lab Report: pGlo Bacterial transformation is a fundamental technique in genetic engineering that allows for the introduction of foreign DNA into bacterial cells. Bing AP Biology 6 February 2013 pGLO Transformation Lab Introduction: The purpose of this lab was to genetically View pGLO Transformation Lab Report. Spin the loop between your index finger and thumb until the entire The document describes a lab experiment that tests how the addition of a pGLO plasmid affects the growth and characteristics of E. Lab 4: pGLO Determining the Presence and Efficiency of Transformation with pGLO and Purifying GFP By Lab Section #, Group #, Date II. 1. txt) or read online for free. pGLO also incorporates a special gene regulation system, which can be Lab report on E. docx from BIOL 3054 at Arkansas Tech University. Pick up the +pGLO tube and immerse the loop into the transformation olution at the bottom of the tube. Selection for cells that have been transformed with pGLO DNA is accomplished by Lab Report: pGlo Bacterial transformation is a fundamental technique in genetic engineering that allows for the introduction of foreign DNA into bacterial cells. pGLO also incorporates a special gene regulation system, which can be used to control . Coli genetic transformation using Green Fluorescent Protein (GFP). This experiment Bio-Rad’s unique pGLO plasmid encodes the gene for GFP and a gene for antibiotic resistance to the antibiotic ampicillin. doc), PDF File (. coli bacteria. Bio-Rad’s unique pGLO plasmid encodes the gene for GFP and a gene for resistance to the antibiotic ampicillin. Plate 1 served as a positive control and showed bacterial Lab Report: pGlo P a g e 2 | 6 Introduction Bacterial transformation is a fundamental technique in genetic engineering that allows for the introduction of foreign DNA into bacterial cells. docx from BIO 181 at Arizona State University, West Campus. doc - Free download as Word Doc (. coli bacteria with a pGLO plasmid. The document summarizes a student laboratory report on transforming E. coli PGLO Transformation Lab Report - Free download as PDF File (. pGLO also incorporates a special gene regulation system, which can be used to control View Lab - pGLO lab report. pdf from BI 113 at Sacred Heart University. High school biology experiment. coli, allowing for the visualization of gene expression through Bio-Rad’s unique pGLO plasmid encodes the gene for GFP and a gene for resistance to the antibiotic ampicillin. coli The document outlines the process of bacterial transformation using the pGLO plasmid to introduce the GFP gene into E. formly circular with smooth edges. Includes procedure, observations, conclusions, and analysis.
